pGLO Lab

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB none none none - pGLO LB/amp none none none + pGLO LB/amp 39 yellow yellow + pGLO LB/amp/ara 31 yellow green

2.	What two new traits do your transformed bacteria have?
	One plate was empty, but there were dots on the two other plates.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	I think there were about two colonies of bacteria because we got only a small amount of it.
4.	What is the role of arabinose in the plates?
	The arabinose activated the gene, allowing it to glow.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	GFP has been used as a reporter gene, a cell maker, and a fusion tag. A reporter gene can be attached to a regulatory sequence of another gene. A fusion tag is a protein.
6.	Give an example of another application of genetic engineering. Genetic engineering is also used to make genetically-modified crops.