I, myself, was absent from the lab, but I was given information from the two people in my group who were present that day. When the gel results were analyzed, the dyes matched with the reference dyes. Our dyes were a bit more circular shaped than the reference dyes which were more rectangular. The two dyes that came out ended up being purple and yellow. A bit different from the reference dyes which happened to be blue, yellow, orange, and red. Since it has a negative charge, I think the fast Green FCF is most similar to my dye. Dog food manufacturers could possibly be adding artificial food colors into dog food to make it more appealing to dog owners who buy it. Maybe the dyes are used to match the meaty flavor and make it seem healthier for the dogs, attracting more dog owners to purchase artificially dyed food. The two factors that control the distance the colored dye solutions migrate are the length and the charge of the dyes. The longer the length, the slower it travels. The shorter the length, the faster it travels. Electricity is the force that helps move the dyes through the gel. The gel lanes and the electric current which runs through the gel are the component of the electrophoresis system that causes the molecules to separate by size. This is because it controls how the dyes move. I would expect DNA molecules with molecular weights of 600, 1000, 2000, and 5000 daltons to separate so that the molecule which weighs 600 would be the first and furthest from the wells. Since 600 is the lowest weight, it would be the fastest. Then the 1000 dalton molecule would be next, then the 2000 dalton molecule, and lastly the 5000 dalton molecule would be the heaviest, causing it to be the slowest and closest to the wells.
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