pGLO Lab

pGLO Observations , Data Recording & Analysis

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Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB none none none - pGLO LB/amp none none none + pGLO LB/amp 39 yellow yellow + pGLO LB/amp/ara 31 yellow green

2.	What two new traits do your transformed bacteria have?
	One plate was empty, but there were dots on the two other plates.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	I think there were about two colonies of bacteria because we got only a small amount of it.
4.	What is the role of arabinose in the plates?
	The arabinose activated the gene, allowing it to glow.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	GFP has been used as a reporter gene, a cell maker, and a fusion tag. A reporter gene can be attached to a regulatory sequence of another gene. A fusion tag is a protein.
6.	Give an example of another application of genetic engineering. Genetic engineering is also used to make genetically-modified crops.

Candy Electrophoresis Lab

I, myself, was absent from the lab, but I was given information from the two people in my group who were present that day.  When the gel results were analyzed, the dyes matched with the reference dyes. Our dyes were a bit more circular shaped than the reference dyes which were more rectangular. The two dyes that came out ended up being purple and yellow. A bit different from the reference dyes which happened to be blue, yellow, orange, and red. Since it has a negative charge, I think the fast Green FCF is most similar to my dye. Dog food manufacturers could possibly be adding artificial food colors into dog food to make it more appealing to dog owners who buy it. Maybe the dyes are used to match the meaty flavor and make it seem healthier for the dogs, attracting more dog owners to purchase artificially dyed food. The two factors that control the distance the colored dye solutions migrate are the length and the charge of the dyes. The longer the length, the slower it travels. The shorter the length, the faster it travels. Electricity is the force that helps move the dyes through the gel. The gel lanes and the electric current which runs through the gel are the component of the electrophoresis system that causes the molecules to separate by size. This is because it controls how the dyes move. I would expect DNA molecules with molecular weights of 600, 1000, 2000, and 5000 daltons to separate so that the molecule which weighs 600 would be the first and furthest from the wells. Since 600 is the lowest weight, it would be the fastest. Then the 1000 dalton molecule would be next, then the 2000 dalton molecule, and lastly the 5000 dalton molecule would be the heaviest, causing it to be the slowest and closest to the wells.

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New Year Goals

One of my goals for 2017 is to consume only healthy foods, such as fruits, vegetables, and complex carbohydrates. I will also only eat home-cooked meals instead of going out to eat. That way, I know every ingredient and seasoning put into my food. First, I will stay strong and not eat any of the girl scout cookies my sister is selling. Next, I will stay away from unhealthy eating environments, such as going out to cheap fast food with my friends. Ultimately, I will eat healthy all throughout 2017, and live a healthy lifestyle.

My second goal for 2017 is to get more sleep. My goal is to get at least 8 hours of sleep every night, as opposed to 6. I will achieve this by having the will power to not click "watch next episode" when watching Netflix, and putting my phone away early in the night. I currently have the horrible habit of sleeping at 12 am and waking up at 6 or 7 am. I will gradually start to sleep earlier and earlier. First, I will aim to sleep at 11 pm. My ultimate goal is to sleep by 10 pm, allowing me to receive 8 hours of sleep if I continue to wake up at 6 am.